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Alternatively, the stock tradition organisms could be grown in an appropriate liquid medium (i.e., Soybean&#a hundred and fifty;Casein Digest Broth or Sabouraud Dextrose Broth) as well as cells harvested by centrifugation, then washed and resuspended in sterile saline TS to obtain a microbial count of about 1 × 108 cfu per mL. [Take note—The